AST Surgical Technologist Exam Practice 2025 – Complete Study Resource

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What degrees Celsius is the steam sterilization biological indicator incubated?

30-35

55-60

The correct temperature range for incubating a steam sterilization biological indicator is between 55 to 60 degrees Celsius. This specific range is chosen because it optimally allows for the growth of the bacterial spores used in the biological indicators, which are typically heat-resistant. Incubating within this range ensures that if the steam sterilization process was effective, any surviving spores are detected, confirming that the sterilization cycle was successful. This is crucial for maintaining patient safety and ensuring that surgical instruments are free of viable microorganisms before use.

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70-75

80-85

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